DNA

Part:BBa_K5319676

Designed by: Wang Xuanhao   Group: iGEM24_ZJU-China   (2024-10-01)


λ peptide-GS linker-vioB

vioB is one of the series of enzymes in the synthesis of purple bacillin. λ peption-GS linker can bind multiple enzymes tightly together with our protein skeleton, thus improving the overall catalytic efficiency of the series of enzymes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 90
    Illegal PstI site found at 775
    Illegal PstI site found at 2017
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 775
    Illegal PstI site found at 2017
    Illegal NotI site found at 992
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2077
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 90
    Illegal PstI site found at 775
    Illegal PstI site found at 2017
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 90
    Illegal PstI site found at 775
    Illegal PstI site found at 2017
    Illegal NgoMIV site found at 931
    Illegal NgoMIV site found at 1845
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None